software colocalization analysis Search Results


software colocalization analysis  (MetaMorph Inc)
90
MetaMorph Inc software colocalization analysis
A pool of myosin VI localizes on the microvillus of intestinal epithelial cells. (A) Immunofluorescence (confocal microscopic) detection of myosin VI expression and localization in Caco-2/Bbe cells and mouse ileum. In Caco-2/Bbe cells, myosin VI is present throughout the brush border, including in the microvillus and in a vesicular pattern in the terminal web and other intracellular compartments (a1–a4). In mouse ileum, myosin VI is present to a lesser extent on the microvillus and in the terminal web (b1–b4). As a negative control, anti-myosin VI antibody was used to show there is no myosin VI staining in ileum from myosin VI KO mice (d) and myosin VI KD Caco-2/Bbe cells (f). (c) wild-type (WT) control; (e) empty-vector-infected control. Scale bar: 20 µm. (B) Analysis of the overlap of myosin VI and protein markers of microvillus by MetaMorph <t>colocalization</t> software. Please note this software shows presence of two proteins in the same intracellular compartment but does not establish physical association. In Caco-2/Bbe cells, ∼46% of myosin VI overlaps with villin, and in mouse ileum ∼9% of myosin VI overlaps with aquaporin 7. For both Caco-2/Bbe cells and mouse ileum, results are mean±s.e.m. of three separate experiments with five or six images analyzed in each experiment (the total number of images analyzed was 16).
Software Colocalization Analysis, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software colocalization analysis/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
software colocalization analysis - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


A pool of myosin VI localizes on the microvillus of intestinal epithelial cells. (A) Immunofluorescence (confocal microscopic) detection of myosin VI expression and localization in Caco-2/Bbe cells and mouse ileum. In Caco-2/Bbe cells, myosin VI is present throughout the brush border, including in the microvillus and in a vesicular pattern in the terminal web and other intracellular compartments (a1–a4). In mouse ileum, myosin VI is present to a lesser extent on the microvillus and in the terminal web (b1–b4). As a negative control, anti-myosin VI antibody was used to show there is no myosin VI staining in ileum from myosin VI KO mice (d) and myosin VI KD Caco-2/Bbe cells (f). (c) wild-type (WT) control; (e) empty-vector-infected control. Scale bar: 20 µm. (B) Analysis of the overlap of myosin VI and protein markers of microvillus by MetaMorph colocalization software. Please note this software shows presence of two proteins in the same intracellular compartment but does not establish physical association. In Caco-2/Bbe cells, ∼46% of myosin VI overlaps with villin, and in mouse ileum ∼9% of myosin VI overlaps with aquaporin 7. For both Caco-2/Bbe cells and mouse ileum, results are mean±s.e.m. of three separate experiments with five or six images analyzed in each experiment (the total number of images analyzed was 16).

Journal: Journal of Cell Science

Article Title: Myosin VI mediates the movement of NHE3 down the microvillus in intestinal epithelial cells

doi: 10.1242/jcs.149930

Figure Lengend Snippet: A pool of myosin VI localizes on the microvillus of intestinal epithelial cells. (A) Immunofluorescence (confocal microscopic) detection of myosin VI expression and localization in Caco-2/Bbe cells and mouse ileum. In Caco-2/Bbe cells, myosin VI is present throughout the brush border, including in the microvillus and in a vesicular pattern in the terminal web and other intracellular compartments (a1–a4). In mouse ileum, myosin VI is present to a lesser extent on the microvillus and in the terminal web (b1–b4). As a negative control, anti-myosin VI antibody was used to show there is no myosin VI staining in ileum from myosin VI KO mice (d) and myosin VI KD Caco-2/Bbe cells (f). (c) wild-type (WT) control; (e) empty-vector-infected control. Scale bar: 20 µm. (B) Analysis of the overlap of myosin VI and protein markers of microvillus by MetaMorph colocalization software. Please note this software shows presence of two proteins in the same intracellular compartment but does not establish physical association. In Caco-2/Bbe cells, ∼46% of myosin VI overlaps with villin, and in mouse ileum ∼9% of myosin VI overlaps with aquaporin 7. For both Caco-2/Bbe cells and mouse ileum, results are mean±s.e.m. of three separate experiments with five or six images analyzed in each experiment (the total number of images analyzed was 16).

Article Snippet: Overlap of myosin VI with the microvillus marker aquaporin 7 was determined with MetaMorph software colocalization analysis.

Techniques: Immunofluorescence, Expressing, Negative Control, Staining, Plasmid Preparation, Infection, Software